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Table 3 Results of RT-qPCR analysis of selected tissue samples groups 10−5 up to 10−9

From: High sensitivity of domestic pigs to intravenous infection with HEV

 

10−5

10−6

10−7

10−8

10−9

T2–25

T2–26

T2–27

T2–28

T2–21

T2–22

T2–23

T2–24

T2–17

T2–18

T2–19

T2–20

T2–13

T2–14

T2–15

T2–16

T2–09

T2–10

T2–11

T2–12

49dpi

63dpi

63dpi

77dpi

77dpi

Faeces

Ct

n.s.

29.5

26.9

23.9

27.1

no Ct

no Ct

no Ct

26.3

32.3

27.1

32.3

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

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n.s.

33.1

249.4

2473.3

163.2

   

283.7

3.5

163.0

3.6

        

Bile

Ct

20.3

21.9

20.8

20.2

27.1

no Ct

no Ct

no Ct

24.3

27.5

24.6

25.1

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

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36,698.1

10,918.1

25,355.3

39,844.7

160.0

   

1269.3

124.9

1006.4

700.5

        

Liver 1

Ct

23.5

23.7

29.4

23.8

23.3

no Ct

no Ct

no Ct

24.4

30.3

no Ct

30.1

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

4016.9

3422.8

41.9

3226.2

1572.7

   

686.2

7.0

 

7.8

        

Liver 2

Ct

23.0

23.5

28.4

25.2

26.0

no Ct

no Ct

no Ct

23.5

30.6

no Ct

32.2

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

5946.9

4025.0

86.6

1115.6

203.2

   

1383.5

5.2

 

1.6

        

Liver 3

Ct

25.3

23.1

29.1

25.2

24.7

no Ct

no Ct

no Ct

23.2

28.0

25.9

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

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1017.0

5475.4

50.6

1120.3

534.1

   

1704.9

40.2

219.8

         

Liver 4

Ct

24.3

22.3

28.2

26.4

22.2

no Ct

no Ct

no Ct

24.1

29.7

26.9

30.3

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

2191.1

10,005.2

103.4

423.1

3978.3

   

882.4

11.3

99.3

7.0

        

Gall bladder

Ct

29.5

30.6

28.1

25.9

29.4

no Ct

no Ct

no Ct

26.2

29.9

24.9

29.1

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

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39.0

15.5

110.0

634.2

14.3

   

171.0

9.5

478.6

17.0

        

Lnn. Hepat

Ct

31.4

29.2

32.6

31.2

30.1

no Ct

no Ct

no Ct

30.9

no Ct

31.5

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

8.5

45.9

3.3

10.0

7.8

   

4.3

 

2.6

         

Spleen

Ct

no Ct

29.3

33.4

31.9

29.1

no Ct

no Ct

no Ct

28.1

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

 

43.9

1.8

5.7

17.4

   

39.0

           

Pancreas

Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

                    

Ln mes

Ct

33.2

32.3

no Ct

no Ct

27.2

no Ct

no Ct

no Ct

30.2

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

2.1

4.4

  

74.5

   

7.4

           

Ln mand

Ct

no Ct

33.2

no Ct

31.4

31.3

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

no Ct

copy

 

2.0

 

8.2

3.2

               
  1. No Ct ≥34.0 (=negative). Viral copy numbers were calculated from CT values determined by RT-qPCR (HEV copies/μl RNA). LN = lymph node