Table 3 The primer sequences of target and internal reference genes used in qRT-PCR
Gene | Forward primer | Reverse primer | PCR products (bp) |
|---|---|---|---|
Erk1 | CTCAGCTTACGACCATGTGC | TCAGGTCCTGCACGATGTAG | 203 |
Erk2 | CTCAGCAACGACCACATCTG | CCAGGCCAAAGTCACAGATC | 151 |
p38 | ACAACATCGTCAAGTGCCAG | CACGTAGCCAGTCATCTCCT | 209 |
JNK | TCAGTCAGTTGAGCACCAGT | ACTTATGCCTGCTCTGCTCA | 229 |
Claudin-1 | CACCCTTGGCATGAAGTGTA | AGCCAATGAAGAGAGCCTGA | 216 |
Claudin-4 | AAGGTGTACGACTCGCTGCT | GACGTTGTTAGCCGTCCAG | 238 |
Occludin | GTTCGACCAATGCTCTCTCAG | CAGCTCCCATTAAGGTTCCA | 200 |
ZO-1 | CGACCAGATCCTCAGGGTAA | AATCACCCACATCGGATTCT | 163 |
GAPDH | GGGTCATCATCTCTGCACCT | GGTCATAAGTCCCTCCACGA | 180 |