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Table 4 Camparision of Mycoplasma bovis recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay and Real-time qPCR assay on clinical samples

From: Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis

Samples Number of samples RPA-LFD Real-time qPCR
Positive Negative Positive rate (%) Positive Negative Positive rate (%)
Nasal swabs 288 72 216 25.00 61 227 21.18
Fresh lungs 80 32 48 40.00 31 49 38.75
Joint fluids 32 4 28 12.50 4 28 12.50
Bulk tanks 42 6 36 14.29 4 38 9.52
Total 442 114 328 25.79 100 342 22.62