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Table 3 Results of Mycoplasma bovis plasmid DNA standards detected by real-time qPCR and recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay

From: Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis

standards DNA (copies/ul) Real-time qPCR RPA-LFD
Ct 1 Ct 2 Ct 3 Test 1 Test 2 Test 3
NC 40.00 40.00 40.00
100 40.00 40.00 40.00 +
101 40.00 37.07 40.00 + + +
102 36.76 36.79 36.41 + + +
103 33.04 32.17 32.04 + + +
104 28.63 28.02 28.30 + + +
105 25.24 25.12 24.94 + + +
106 20.30 20.76 20.71 + + +
107 17.53 17.52 17.29 + + +
  1. NC negative control (DNase-free water)