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Table 3 Results of Mycoplasma bovis plasmid DNA standards detected by real-time qPCR and recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay

From: Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis

standards DNA (copies/ul)

Real-time qPCR

RPA-LFD

Ct 1

Ct 2

Ct 3

Test 1

Test 2

Test 3

NC

40.00

40.00

40.00

100

40.00

40.00

40.00

+

101

40.00

37.07

40.00

+

+

+

102

36.76

36.79

36.41

+

+

+

103

33.04

32.17

32.04

+

+

+

104

28.63

28.02

28.30

+

+

+

105

25.24

25.12

24.94

+

+

+

106

20.30

20.76

20.71

+

+

+

107

17.53

17.52

17.29

+

+

+

  1. NC negative control (DNase-free water)