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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis

Fig. 4

Comparison of sensitivities of the recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) and Real-time qPCR assays. Molecular sensitivity test results of the two assays were assessed using 10-fold serially diluted DNA as template. a Results by Real-time qPCR (with a detection limit of 83 copies/reaction DNA standards). b Results by RPA-LFD (with a detection limit of 20 copies/reaction DNA standards). Lane 1 to 8: 10-fold serially diluted Mycoplasma bovis plasmid DNA standards from 107 to 100 copies/uL. Lane NC: negative control (DNase-free water)

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