Fig. 3From: Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovisOptimization of incubation temperature and reaction time for Mycoplasma bovis recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay. a The amplification performance of RPA-LFD was effectively within the range of 35 °C to 45 °C. b Determination of amplification time. After 10 min of amplification reaction, the test line was clearly visible on the strip. Lane NC: negative control (DNase-free water). Samples were tested in triplicate with one reaction displayed in figure for each triplicateBack to article page