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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis

Fig. 2

Screening of Mycoplasma bovis recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) primers and probe. a: The results of RPA-nfo reaction were detected by agarose-gel electrophoresis from seven set of primers and probe combination; b: ‘a’ showed results of RPA-nfo reaction by LFD test, and DNA template came from extracted Mycoplasma bovis reference type strain PG45 colonies, and ‘b’ was negative control (DNase-free water) that the corresponding combination of primers and probe. Samples were tested in triplicate with one reaction displayed in figure for each triplicate

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