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Table 1 Reproducibility testing of Bicolor FMCA for each positive control sample

From: Duplex fluorescence melting curve analysis as a new tool for rapid detection and differentiation of genotype I, II and Bartha-K61 vaccine strains of pseudorabies virus

Name

No. of times tested

Tm value (°C, Mean ± 3SD)

P1 (FAM channel)

P2 (HEX channel)

Bartha-K61

32

70.41 ± 0.49 a,*

71.62 ± 0.18*

NIA3

31

76.00 ± 0.48b

HDDJ

32

76.93 ± 0.48

  1. aTm value calculated based on the number of Bartha-K61 strains and NIA3 strains tested in the FAM channel. bTm value calculated based on the number of NIA3 strains and HDDJ strains tested in the HEX channel. *: The asterisk symbol was indicated significant difference between the melting temperature of two genotypes in same channel (P<0.001).
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BMC Veterinary Research

ISSN: 1746-6148

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