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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Duplex fluorescence melting curve analysis as a new tool for rapid detection and differentiation of genotype I, II and Bartha-K61 vaccine strains of pseudorabies virus

Fig. 3

Simultaneous detection of PRV Bartha-K61vaccine and Genotype II strains in mixed infections. Two co-existing genotypes were detected in the FAM channel (a) and the HEX channel (b). Melting curves of artificial plasmid templates containing p-B and p-H at various ratios (10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9, 0:10) were tested. The overall template concentration was 108 copies per reaction. The NTC negative control is represented by a grey line

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BMC Veterinary Research

ISSN: 1746-6148

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