Fig. 2From: Duplex fluorescence melting curve analysis as a new tool for rapid detection and differentiation of genotype I, II and Bartha-K61 vaccine strains of pseudorabies virusSpecificity of the duplex FMCA method. Melting curves obtained from duplex FMCA in the FAM channel (a) and the HEX channel (b) with 12 samples comprising 3 PRV positive samples, and five other viruses known to cause similar clinical symptoms in pig. Reference recombinant plasmids p-B (Bartha-K61 vaccine), p-N (Genotype I), and p-H (Genotype II) served as positive controls corresponding to red, blue, and green lines, and a No Template Control (NTC) served as the negative control (grey line)Back to article page