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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Duplex fluorescence melting curve analysis as a new tool for rapid detection and differentiation of genotype I, II and Bartha-K61 vaccine strains of pseudorabies virus

Fig. 2

Specificity of the duplex FMCA method. Melting curves obtained from duplex FMCA in the FAM channel (a) and the HEX channel (b) with 12 samples comprising 3 PRV positive samples, and five other viruses known to cause similar clinical symptoms in pig. Reference recombinant plasmids p-B (Bartha-K61 vaccine), p-N (Genotype I), and p-H (Genotype II) served as positive controls corresponding to red, blue, and green lines, and a No Template Control (NTC) served as the negative control (grey line)

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