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Table 4 Effect of weaning stress on the relative gene expression of Fas, IFN-γ, NFκB1, NFκB2, CD62L

From: An intensive milk replacer feeding program benefits immune response and intestinal microbiota of lambs during weaning

Variable

Days post weaning1

P-values3

Group

0

1

2

3

7

14

21

SEM

T2

S

T × S

Fas

C

3.52

3.13

3.13

3.00

3.65

4.51a

3.42

0.14

NS

**

NS

 

H

3.14

3.32

2.65

3.34

3.73

4.07

3.37

0.15

IFN-γ

C

1.85

2.60a,x

2.48

2.58a,x

3.20c,x

2.38

1.73

0.12

**

*

NS

 

H

1.36

1.74y

2.04

1.44y

1.82y

1.81

1.71

0.10

NFκB1

C

2.68

2.69

1.83a

3.55a

3.65a

4.82c

4.16c

0.16

NS

***

NS

 

H

2.82

3.11

1.24c

3.74a

2.94

4.54c

3.81a

0.19

NFκB2

C

2.54

2.06

2.16

2.47x

2.30

2.63

4.54a

0.15

*

***

*

 

H

2.36

2.15

1.93

1.09c,y

2.23

2.54

3.99c

0.13

CD62L

C

1.86

4.01c

3.34c,x

2.41x

3.00a

3.05b

2.48

0.14

*

***

**

 

H

1.80

3.17b

1.80y

1.35y

2.25

3.17b

3.27c

0.16

  1. Values are expressed as least squares means (LS-means)
  2. 11, 2, 3, 7, 14, and 21 d relative to weaning (= 0 d)
  3. 2T: MR treatment; S: sampling time; T × S: MR treatment × sampling time interaction
  4. 3Superscripts a, b, c within rows to indicate the LS-means differ from 1, 2, 3, 7, 14, and 21 d compared with 0 d by P < 0.05, P < 0.01, and P < 0.001, respectively; x, y within columns, to indicate that the LS-means differ from C and H groups by P < 0.05; * P < 0.05, ** P < 0.01, *** P < 0.001, NS: not significant (P > 0.05)