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Table 3 Effect of weaning stress on the relative gene expression of CXCL8, IL-1β, GRα, TLR4, TNFα

From: An intensive milk replacer feeding program benefits immune response and intestinal microbiota of lambs during weaning

Variable Days post weaning1 P-values3
Group 0 1 2 3 7 14 21 SEM T2 S T × S
CXCL8 C 1.65 3.16c 3.27c 4.34c,x 4.11c,x 4.13c,x 3.52c,x 0.15 ** *** ***
  H 2.46 2.69 2.97 2.18y 2.09y 2.41y 2.34y 0.12
IL-1β C 1.89 2.09x 1.77x 3.22c,x 3.17b,x 4.22c 5.40c,x 0.20 ** *** ***
  H 2.68 3.37y 4.11c,y 5.37c,y 4.70c,y 4.77c 4.04c,y 0.16
GRα C 1.53 2.72c 4.92c 5.94c,x 6.97c,x 6.70c,x 3.63c 0.28 *** *** ***
  H 1.06 2.07b 4.50c 4.30c,y 3.17c,y 3.57c,y 3.58c 0.18
TLR4 C 2.55 2.36x 2.94x 3.02x 3.63b 4.43c,x 3.84b 0.14 *** *** ***
  H 3.00 4.04b,y 5.50c,y 4.48c,y 3.06 2.60y 3.12 0.16
TNFα C 2.63 3.80c 7.29c,x 7.96c,x 6.74c,x 2.79x 2.90 0.32 *** *** ***
  H 2.42 3.22 3.69b,y 2.96y 2.57y 3.73b,y 2.39 0.13
  1. Values are expressed as least squares means (LS-means)
  2. 11, 2, 3, 7, 14, and 21 d relative to weaning (= 0 d)
  3. 2T: MR treatment; S: sampling time; T × S: MR treatment × sampling time interaction
  4. 3Superscripts a, b, c within rows to indicate the LS-means differ from 1, 2, 3, 7, 14, and 21 d compared with 0 d by P < 0.05, P < 0.01, and P < 0.001, respectively; x, y within columns, to indicate that the LS-means differ from C and H groups by P < 0.05; * P < 0.05, ** P < 0.01, *** P < 0.001