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Table 2 Effect of weaning stress on leukocytes, red blood cell number (RBC), neutrophil: lymphocyte ratio, and hemoglobin concentration (HGB)

From: An intensive milk replacer feeding program benefits immune response and intestinal microbiota of lambs during weaning

Variable   Days post weaning1    P-values3
Group 0 1 2 3 7 14 21 SEM T2 S T × S
Total leukocytes (× 109cells/L) C 8.56 10.58c 9.76a 9.28 9.39 9.18 8.61 0.18 NS ** NS
  H 8.42 9.52a 8.94 9.09 9.25 9.07 8.90 0.14
Lymphocytes (×109 cells/L) C 3.44 4.12 3.68 3.95 4.44a 4.50a 3.80 0.13 NS * NS
  H 3.57 3.87 4.21 3.97 4.58a 4.08 3.66 0.12
Neutrophils (×109 cells/L) C 3.68 5.14c,x 4.62a,x 4.06 4.37 3.69 3.26 0.14 NS ** 0.026
  H 3.39 3.97y 3.71y 4.21a 3.72 3.96 3.81 0.10
N:L ratio C 1.07 1.32 1.31x 1.04 1.00 0.83 0.90 0.05 NS NS NS
  H 0.99 1.06 0.94y 1.14 0.86 1.01 1.17 0.04
RBC (× 1012 cells/L) C 8.20 8.27 7.96x 7.93x 7.95x 8.20x 8.49 0.07 *** *** ***
  H 8.09 8.66b 8.85c,y 8.85c,y 8.55a,y 8.66b,y 9.81c,y 0.08
HGB (g/L) C 114.09 115.96x 108.59x 107.96x 103.96bx 111.09x 120.34x 1.44 *** *** **
  H 116.29 124.54a,y 126.91b,y 127.16b,y 120.79y 122.29y 138.16c,y 1.13
  1. Values are expressed as least squares means (LS-means)
  2. 11, 2, 3, 7, 14, and 21 d relative to weaning (= 0 d)
  3. 2T: MR treatment; S: sampling time; T × S: MR treatment × sampling time interaction
  4. 3Superscripts a, b, c within rows to indicate the LS-means differ from 1, 2, 3, 7, 14, and 21 d compared with 0 d by P < 0.05, P < 0.01, and P < 0.001, respectively; x, y within columns, to indicate that the LS-means differ between the C and H groups by P < 0.05; * P < 0.05, ** P < 0.01, *** P < 0.001, NS: not significant (P > 0.05)