From: Listeriosis in fattening pigs caused by poor quality silage - a case report
 |  | Pig 1 | Pig 2 | Silage |
---|---|---|---|---|
Pathoanatomical and pathohistological findings | thorax | low grade dystelectasis, low grade peribronchial interstitial inflammatory round cell infiltration | high-grade pulmonary oedema, low grade peribronchial interstitial inflammatory round cell infiltration | n.a. |
abdomen | severe diffuse fibrinonecrotic typhlocolitis, remaining abdominal organs (including inguinal lymphnodes) without pathological findings | severe diffuse fibrinonecrotic typhlocolitis; moderate diffuse fibrinous serositis in the area of the intestine | n.a. | |
IHC L. monocytogenes | positive in: lymphatic tissue, lamina muscularis mucosae and lamina propria mucosae of the large intestine; spleen, inguinal lymph nodes | n.a. | ||
Triplex-PCR | positive (B. hyodysenteriae) | negative | n.a. | |
Microbiological examination of serosa | L. monocytogenes (Pig 1 + 2; Silage) | |||
PFGE analysis of L. monocytogenes | PCR-serogroup 1/2a, 3a (Pig 1 + 2; Silage) | |||
MLST of L. monocytogenes isolates | ST21 (Pig 1 + 2; Silage) | |||
AST of L. monocytogenes isolates | AMP (0.12 to 16 μg/ml)a | sensitive (≤0.12)b | sensitive (≤0.12)b | sensitive (≤0.12)b |
PEN (0.06 to 8Â ÎĽg/ml)a | sensitive (0.25)b | sensitive (0.12)b | sensitive (0.1)b | |
SXT (0.5/9.5 to 4/76 μg/ml)a | sensitive(≤0.5/≤9.5)b | sensitive(≤0.5/≤9.5)b | sensitive(≤0.5/≤9.5)b |