Table 3 Sequences of PCR primers used in this study
Gene | Forward (5′-3′) | Reverse (5′-3′) | Reference |
|---|---|---|---|
fGAPDH | ACGATGACATCAAGAAGGTG | CACACCAGGAAATGAGCTTG | [51] |
fFOXP3 | GCCTGCCACCTGGAATCAAC | GTGTGCTGGGGCTTGGGA | [52] |
fIFN-γ | TACACAAGTTTTATTTTCGCTTTCC | TGCTACATCTGGATTACTTGCATTA | [51] |
fIL-6 | TGAAAAAGGAGATGTGTGACAACTA | CCTGAAGACCAGTAGTGATTCTTGT | [51] |
fIL-10 | CCTTTAGTAAGCTCCAAGAGAAAGG | CAGATTTTCATCTTCATTGTCATGT | [51] |
fTNF-α | GACACTCAGATCATCTTCTCGAACT | GACCTGGGAGTAGATGAGGTACAG | [51] |
fIL-1β | CATACAGTCACAGGACTACACGTTC | TTGATGCACAACACTACTGGTATCT | This study |
fIL-4 | GGCAGATCTATACACATCACAACTG | GCTTTGAGTATTTCTTTTGCATGAT | This study |
mGAPDH | TCATTGACCTCAACTACAA | ACACCAGTAGACTCCACGT | [51] |
mINF-γ | CACAGTCATTGAAAGCCTAGAAAGT | AGTTCCTCCAGATATCCAAGAAGAG | This study |
mIL-6 | CGCACTAGGTTTGCCGAGTA | CCTTTCTACCCCAATTTCCA | This study |
mIL-10 | GTGATTTTAATAAGCTCCAAGACC | GATCATCATGTATGCTTCTATGCAG | This study |
mTNF-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG | [51] |
mIL-1β | GTCTTTCCCGTGGACCTTC | TGTTCATCTCGGAGCCTGT | [51] |
mIL-4 | TAGTTGTCATCCTGCTCTTCTTTCT | CGATGATCTCTCTCAAGTGATTTTT | This study |
fCOX-2 | CGATTCAGTCTCTCATCTGCAATAA | TCAGTTGAACGTTCTTTTAGCAGTA | [51] |