Skip to main content

Table 3 Sequences of the primers, probes and reference genes used for the duplex real-time PCR assay

From: Transcripts of antibacterial peptides in chicken erythrocytes infected with Marek’s disease virus

Primer/probe name

Primer/probe sequence(5′ → 3′)

Target gene

GenBank Accession number

meq-F

GGAGCCGGAGAGGCTTTATG

  

meq-R

ATCTGGCCCGAATACAAGGAA

MDV Eco Q

M89471

meq-P

(FAM)CGTCTTACCGAGGATCCCGAACAGG(TAMRA)

  

ovo-F

CACTGCCACTGGGCTCTGT

  

ovo-R

GCAATGGCAATAAACCTCCAA

ovotransfenrin

Y00407

ovo-P

(ROX)AGTCTGGAGAAGTCTGTGCAGCCTCCA(BHQ2)

  
  1. FAM: Carboxy fluorescein, TAMRA: Carboxy tetramethyl rhodamine, ROX: Carboxy-X-rhodamine, BHQ2: Black Hole Quencher 2