Methionine to cystine ratio in the total sulfur amino acid requirements and sulfur amino acid metabolism using labelled amino acid approach for broilers

Pacheco, Letícia G.; Sakomura, Nilva K.; Suzuki, Rafael M.; Dorigam, Juliano C. P.; Viana, Gabriel S.; Van Milgen, Jaap; Denadai, Juliana C.

doi:10.1186/s12917-018-1677-8

Table 7 Ingredients and nutrient composition of experimental diets as-fed basis

From: Methionine to cystine ratio in the total sulfur amino acid requirements and sulfur amino acid metabolism using labelled amino acid approach for broilers

Met:Cys/TSAA TSAAratio	Standart diet (1-14d)	44:56	47:53		50:50		53:47		56:44
Ingredients (g.kg⁻¹)	Standart diet (1-14d)	No Met	L-Met	DL-Met	L-Met	DL-Met	L-Met	DL-Met	L-Met	DL-Met
Corn (7.86%)	620.64	545.60	545.04	545.59	544.47	545.58	543.91	545.56	543.34	545.55
Soybean meal (45%)	216.68	350.03	350.10	350.03	350.17	350.03	350.24	350.03	350.31	350.04
Corn gluten meal (60%)	105.11
Peanult meal (48.2%)		20.93	21.24	20.99	21.55	21.05	21.86	21.10	22.18	21.16
Soybean oil	9.27	45.81	46.00	45.77	46.19	45.73	46.37	45.68	46.56	45.64
Dicalcium phosphate	19.39	15.27	15.26	15.27	15.26	15.27	15.26	15.27	15.26	15.26
Limestone	9.31	9.04	9.04	9.04	9.04	9.04	9.04	9.04	9.04	9.04
Sodium bicarbonate	2.00	4.14	4.14	4.14	4.13	4.14	4.13	4.14	4.13	4.14
Salt	3.61	2.08	2.08	2.08	2.08	2.08	2.08	2.08	2.08	2.08
DL-Methionine (98%)	2.88	0.00	0.00	0.19	0.00	0.37	0.00	0.55	0.00	0.74
L-Methionine (99%)		0.00	0.18	0.00	0.37	0.00	0.55	0.00	0.73	0.00
L-Cystine (100%)		0.73	0.55	0.55	0.37	0.37	0.18	0.18	0.00	0.00
L-Lysine HCl (78%)	7.03	2.37	2.37	2.37	2.37	2.37	2.37	2.37	2.37	2.37
L-Threonine (98%)	1.58	0.93	0.93	0.93	0.93	0.93	0.93	0.93	0.93	0.93
L-Valine (96.5%)		0.47	0.47	0.47	0.47	0.47	0.48	0.47	0.48	0.47
Premix^a	1.5	1.60	1.60	1.60	1.60	1.60	1.60	1.60	1.60	1.60
Choline Chloride (60%)	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
Nutrients content
Met. energy (Mcal/Kg)	3.100	3.10 (2.9)	3.10 (2.9)	3.10 (2.9)	3.10 (2.9)	3.10 (2.9)	3.10 (2.9)	3.10 (2.9)	3.10 (2.9)	3.10 (2.9)
Crude protein (%)	22.0	21.5 (22)	21.5 (22)	21.5 (22)	21.5 (22)	21.5 (22)	21.5 (22)	21.5 (22)	21.5 (22)	21.5 (22)
Dig. Met+Cys (%)	0.953	0.65 (0.60)	0.65 (0.60)	0.65 (0.62)	0.65 (0.60)	0.65 (0.60)	0.65 (0.61)	0.65 (0.62)	0.65 (0.61)	0.65 (0.60)
Dig. Met (%)	0.639	0.29 (0.26)	0.31 (0.28)	0.31 (0.29)	0.33 (0.30)	0.33 (0.30)	0.34 (0.32)	0.34 (0.33)	0.36 (0.34)	0.36 (0.34)
Dig. Cys (%)	0.314	0.36 (0.34)	0.34 (0.32)	0.34 (0.33)	0.33 (0.30)	0.33 (0.30)	0.31 (0.29)	0.31 (0.29)	0.29 (0.27)	0.29 (0.26)
Dig. Lys (%)	1.324	1.19 (1.16)	1.19 (1.16)	1.19 (1.16)	1.19 (1.16)	1.19 (1.16)	1.19 (1.16)	1.19 (1.16)	1.19 (1.16)	1.19 (1.16)
Dig. Thr (%)	0.861	0.79 (0.75)	0.79 (0.75)	0.79 (0.75)	0.79 (0.75)	0.79 (0.75)	0.79 (0.75)	0.79 (0.75)	0.79 (0.75)	0.79 (0.75)
Dig. Val (%)	1.019	0.92 (0.89)	0.92 (0.89)	0.92 (0.89)	0.92 (0.89)	0.92 (0.89)	0.92 (0.89)	0.92 (0.89)	0.92 (0.89)	0.92 (0.89)

^a Content (per kg of diet) – vit. A = 10,575 UI; vit. D3 = 2554 UI; vit. K = 1.8 mg; vit. E = 14.87 mg; vit. B1 = 2.00 mg; vit. B2 = 4.5 mg; vit. B6 = 2.50 mg; vit. B12 = 2.00 mg; niacin = 30.00 mg; folic acid = 0.75 mg; calcium pantothenate = 11.74 mg; biotin = 0.01; iron = 43.44 mg; zinc = 43.35 mg; copper = 8.56 mg; manganese = 56.00 mg; iodine = 0,56 mg; selenium = 0,34 mg; antioxidant 4.20 mg; Salinomycin sodium 12%; Butil hidroxy toluene BHT. Values in parentheses indicate analyzed dietary concentration of the amino acids

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