Table 1 Information about the real-time PCR primers used to measure mRNA transcript levels of various cytokines
Genes | Forward Primer (5′- 3′) | Reverse Primer (5′- 3′) | Accession/Reference |
|---|---|---|---|
β-Actin | GCGGGACATCAAGGAGAAG | AGGAAGGAGGGCTGGAAGAG | U07786 |
IFN-α | TCTCATGCACCAGAGCCA | CCTGGACCACAGAAGGGA | [39] |
IFN-β | AGTGCATCCTCCAAATCGCT | GCTCATGGAAAGAGCTGTGGT | M86762 |
TNF-α | TTATTCAGGAGGGCGAGGT | AGCAAAAGGAGGCACAGAGG | NM214022 |
IL-1α | GTGCTCAAAACGAAGACGAACC | CATATTGCCATGCTTTTCCCAGAA | NM_214029.1 |
IL-1β | AACGTGCAGTCTATGGAGT | GAACACCACTTCTCTCTTCA | M86725 |
IL-6 | CCACCAGGAACGAAAGAGAG | AGGCAGTAGCCATCACCAGA | NM_214399 |
IL-8 | TAGGACCAGAGCCAGGAAGA | CAGGAAAACTGCCAAGAAGG | M86923.1 |
IL-10 | TGACGATGAAGATGAGGAAGAA | GAACCTTGGAGCAGATTTTGA | NM214041 |
IL-12 | TCAGGGACATCATCAAACCA | GAACACCAAACATCAGGGAAA | NM214013 |
IFN-γ | GACTTTGTGTTTTTCTGGCTCTTAC | TTTTGTCACTCTCCTCTTTCCA | NM213948 |
IL-2 | TGCACTAACCCTTGCACTCA | CCTGCTTGGGCATGTAAAAT | X56750 |
IL-4 | TTTGCTGCCCCAGAGAAC | TCCTGTCAAGTCCGCTCA | X68330 |