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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Fibroblast growth factor-1 as a mediator of paracrine effects of canine adipose tissue-derived mesenchymal stem cells on in vitro-induced insulin resistance models

Fig. 2

Insulin resistance was induced in 3T3-L1 adipocytes in vitro. a Adipocyte differentiation of 3T3-L1 cells and following induction of insulin resistance were summarized with the course of time. b Fibroblast-like 3T3-L1 pre-adipocytes at day 0. c Differentiating 3T3-L1 cells at day 3. d Differentiated 3T3-L1 adipocytes containing cytoplasmic lipid droplets at day 9. Original magnification: 200x. e, f Alterations in mRNA expression levels of IRS-1 and GLUT4 during the differentiation and insulin resistance induction were shown. Results are presented as mean ± SD obtained from three independent experiments *P < 0.05, **P < 0.01

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