Skip to main content

Table 2 Primers used for the quantitative RT-PCR

From: Protective immunity induced by Eimeria common antigen 14–3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima

RNA target

Primer sequence

Accession NO.

Amplification efficiency (%)a

Correlation coefficients (r2)

IFN-γ

Forward: 5′-AGCTGACGGTGGACCTATTATT-3′

Y07922

99.16

0.9976

Reverse: 5′-GGCTTTGCGCTGGATTC-3′

IL-2

Forward: 5′-TCTGGGACCACTGTATGCTCT-3′

AF000631

98.53

0.9932

Reverse: 5′-ACACCAGTGGGAAACAGTATCA-3’

TNFSF15

Forward:5′-CCTGAGTTATTCCAGCAACGCA-3′

NM_001024578

98.51

0.9992

Reverse: 5′-ATCCACCAGCTTGATGTCACTAAC-3′

IL-17D

Forward:5′-GCTGCCTCATGGGGATCTTTGGTG-3′

EF570583

98.18

0.9954

Reverse: 5′-CGATGACGGCTTGTTCTGGTTGAC-3′

TGF-β4

Forward: 5′-CGGGACGGATGAGAAGAAC-3′

M31160

97.39

0.9981

Reverse: 5′-CGGCCCACGTAGTAAATGAT-3′

IL-4

Forward: 5′-ACCCAGGGCATCCAGAAG-3′

AJ621735

99.41

0.9996

Reverse: 5′-CAGTGCCGGCAAGAAGTT-3′

GAPDH

Forward: 5′-GGTGGTGCTAAGCGTGTTAT-3′

K01458

95.48

0.9994

Reverse: 5′-ACCTCTGTCATCTCTCCACA-3′

  1. aAmplification efficiency (%) = (10–1/slope − 1) × 100