Table 4 Primer pairs used in this study
From: A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses
Targeted virus | Forward primers (5′-3′) | Reverse primers (5′-3′) | Amplicon size (bp) | Bead region | Source |
|---|---|---|---|---|---|
CAV | ATACTTTACAAACAAATAACACAC-spacer18-GCTCTCCAAGAAGATACTCCA | GCATTCGCGCAGCCACAC | 149 | 19 | this study |
MDV | TACTTAAACATACAAACTTACTCA-spacer18-CCCATTCCCTCTTCTGCC | GCTGAGCGTAAACCGTC | 114 | 65 | this study |
IBDV | TACTTCTTTACTACAATTTACAAC-spacer18-ATGCGGAGCCTTCTGA | ATTAGCCCTGACCCTGTG | 128 | 15 | this study |
ARV | ACTTATTTCTTCACTACTATATCA-spacer18-GGATTCCGTCTCCATTCT | GAGTTTCCGTCAACCGTA | 102 | 34 | this study |
REV | CTTAAACTCTACTTACTTCTAATT-spacer18-GACTGCCTTGTGACTGCT | ACTCCCACTGTTGTCTAAATC | 153 | 56 | this study |
CAV | GACTGTAAGATGGCAAGACGAGCTC | GGCTGAAGGATCCCTCATTC | 675 | / | Caterina et al., 2004 [17] |
IBDV | AGCCTTCTGATGCCAACAAC | ATCTGTCAGTTCACTCAGGC | 365 | / | Caterina et al., 2004 [17] |
ARV | GGTGCGACTGCTGTATTTGGTAAC | AATGGAACGATAGCGTGTGGG | 532 | / | Caterina et al., 2004 [17] |
MDV | GTGATGGGAAGGCGATAGAA | TCCGCATATGTTCCTCCTTC | 225 | / | Cao et al., 2013 [30] |
REV | AATGGTTGTAAAGGGCAGAT | CTCCTCTCACTGCCAATCT | 200 | / | Cao et al., 2013 [30] |