Table 1 The average of the MFI values with SD of sensitivity test by the xTAG assay and conventional PCR/RT-PCR
From: A multiplex xTAG assay for the simultaneous detection of five chicken immunosuppressive viruses
Sample (copies/ul) | IBDV | CAV | ARV | REV | MDV | |||||
|---|---|---|---|---|---|---|---|---|---|---|
xTAG assay | xTAG assay | xTAG assay | xTAG assay | xTAG assay | ||||||
MFI values ±SD | RT-PCRP/N | MFI values ±SD | RT-PCRP/N | MFI values ±SD | RT-PCRP/N | MFI values ±SD | RT-PCRP/N | MFI values ±SD | RT-PCRP/N | |
108 | 8035 ± 178 | + | 11,687.5 ± 574 | + | 12,380 ± 382 | + | 8742.5 ± 191 | + | 9143.5 ± 172 | + |
107 | 7764 ± 84 | + | 10,063.5 ± 279 | + | 10,208 ± 465 | + | 9302 ± 170 | + | 8702 ± 152 | + |
106 | 6782 ± 138 | + | 8503 ± 191 | + | 5944 ± 119 | + | 7470.5 ± 310 | + | 7496 ± 169 | + |
105 | 5380.5 ± 110 | + | 8567 ± 185 | + | 4803 ± 135 | + | 5938 ± 97 | + | 4350 ± 198 | + |
104 | 4034 ± 230 | + | 5107.5 ± 72 | + | 4277 ± 382 | + | 4233 ± 134 | + | 3227 ± 157 | + |
103 | 1402 ± 38 | + | 2680.5 ± 122 | + | 1683 ± 347 | + | 1358.5 ± 182 | + | 1806.5 ± 220 | + |
102 | 215 ± 184 | – | 767.5 ± 206 | + | 1121 ± 26 | + | 924 ± 37 | + | 1039 ± 128 | + |
101 | 167 ± 138 | – | 191.5 ± 139 | – | 137 ± 38 | – | 141.5 ± 46 | – | 138.5 ± 59 | – |
blank | 78 ± 45 | – | 89 ± 17 | – | 48 ± 35 | – | 128 ± 83 | – | 85 ± 21 | – |