Fig. 1

Result of the specificity testing. Viral target specificity was tested in a multiplex mode using target-specific primers. The targets of IBDV, CAV, ARV, REV and MDV were tested against each other and the non-target viral pathogens: avian influenza virus (AIV), infectious bronchitis virus (IBV), newcastle disease virus (NDV), infectious laryngotracheitis virus (ILTV), Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). The cut-off value was 600, defined as the mean of the net MFI from negative PCR controls with three times of standard deviation (MFI +3SD). All the samples were performed in triplicate