Interrelationship between tetracycline resistance determinants, phylogenetic group affiliation and carriage of class 1 integrons in commensal Escherichia coli isolates from cattle farms

Table 1 Primers used for the PCR detection of resistance genes

Primer name	Target gene	Nucleotide sequence	Annealing temperature (°C)	Amplicon size (bp)	Reference
TetA-F	tet(A)	GGCGGTCTTCTTCATCATGC	55	502	[21]
TetA-R	tet(A)	CGGCAGGCAGAGCAAGTAGA	55	502	[21]
TetB-F	tet(B)	CATTAATAGGCGCATCGCTG	55	930	[21]
TetB-R	tet(B)	TGAAGGTCATCGATAGCAGG	55	930	[21]
TetC-F	tet(C)	GCTGTAGGCATAGGCTTGGT	55	888	[21]
TetC-R	tet(C)	GCCGGAAGCGAGAAGAATCA	55	888	[21]
TetD-F	tet(D)	GAGCGTACCGCCTGGTTC	55	780	[12]
TetD-R	tet(D)	TCTGATCAGCAGACAGATTGC	55	780	[12]
TetE-F	tet(E)	AAACCACATCCTCCATACGC	55	278	[22]
TetE-R	tet(E)	AAATAGGCCACAACCGTCAG	55	278	[22]
TetG-F	tet(G)	GCTCGGTGGTATCTCTGCTC	55	468	[23]
TetG-R	tet(G)	AGCAACAGAATCGGGAACAC	55	468	[23]
Sul1-F	sul1	CGGCGTGGGCTACCTGAACG	57	433	[24]
Sul1-R	sul1	GCCGATCGCGTGAAGTTCCG	57	433	[24]
Sul2-F	sul2	CGGCATCGTCAACATAACCT	57	721	[21]
Sul2-R	sul2	TGTGCGGATGAAGTCAGCTC	57	721	[21]
Sul3-F	sul3	CAACGGAAGTGGGCGTTGTGGA	57	244	[25]
Sul3-R	sul3	GCTGCACCAATTCGCTGAACG	57	244	[25]
Cat-F	Cat	GGT GAG CTG GTG ATA TGG	55	209	[26]
Cat-R	Cat	GGG ATT GGC TGA GAC GA	55	209	[26]
Flor -F	flor	CAC GTT GAG CCT CTA TAT	55	868	[27]
Flor -R	flor	ATG CAG AAG TAG AAC GCG	55	868	[27]
CmlA -F	cmlA	TGT CAT TTA CGG CAT ACT CG	55	455	[27]
CmlA -F	cmlA	ATC AGG CAT CCC ATT CCC AT	55	455	[27]
Var1-F	var1	GGCATCCAAGCAGCAAG	55	Variable	[28]
Var1-R	var1	AAGCAGACTTGACCTGA	55	Variable	[28]
qacEΔ1 F	qacEΔ1	ATCGCAATAGTTGGCGAAGT	60	225	[29]
qacEΔ1 R	qacEΔ1	CAAGCTTTTGCCCATGAAGC	60	225	[29]
IntI1-F	intI1	GGGTCAAGGATCTGGATTTCG	60	483	[30]
IntI1-R	intI1	ACATGCGTGTAAATCATCGTCG	60	483	[30]

ISSN: 1746-6148