Fig. 5
Effect of serum treatment on porcine pGFAP-CreERT2 NSCs. a Phase contrast image of porcine pGFAP-CreERT2 NSCs with or without 10% FBS treatment. Scale, 50 μm. b Total cell population doublings estimated by cell counting at each passage. Independent experiments were replicated three times. c Representative confocal images showing the expression of astrocytic GFAP and neuronal TUJ1 after differentiation. Some cells revealed a strong GFAP expression (white arrow) or TUJ1 expression (dashed white arrow) in FBS treated groups. Scale, 100 μm