Fig. 4

Spontaneous differentiated cells from porcine pGFAP-CreER^T2 NSCs. a Brightfield image of pGFAP-CreER^T2 neurospheres cultured in maintenance or differentiation media for 10 days. Scale, 200 μm. b Representative image of the formation neural network (left). Phase contrast image of differentiated pGFAP-CreER^T2 cells at a higher magnification (right). Scale, 50 μm c Immunofluorescence analysis for astrocyte GFAP, neuronal TUJ1, and NSC Nestin marker expression in differentiated cells at 2 weeks. Scale, 50 μm