Fig. 2From: Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic pigletCharacterization of porcine pGFAP-CreERT2 NSCs. a Karyotype analysis of pGFAP-CreERT2 NSCs. b Immunofluorescence analysis of the neural stem cell markers Nestin, Sox2, and GFAP in pGFAP-CreERT2 NSCs. Scale, 100 μm. c Primary neurosphere-generating cells from adult porcine pGFAP-CreERT2 brain tissue cultured in the presence of EGF and FGF2. Scale, 100 μm. d Quantification analysis of primary, secondary, and tertiary sphere formation from cells obtained from adult porcine pGFAP-CreERT2 neocortex or SVZ. Neurosphere number was determined 10 days after plating. Data on the neurosphere number are the average ± SEM of 4 independent experiments consisting of 3 replicates. *P < 0.05Back to article page