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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic piglet

Fig. 1

Isolation of primary cells from brain tissue explants of pGFAP-CreERT2 piglets. (A) Schematic of the vectors used in this experiment. (B) Representative image of pGFAP-CreERT2 fibroblast donor cells. Scale, 100 μm (a), pGFAP-CreERT2 nuclear transfer (NT) blastocysts. Scale, 200 μm (b), pGFAP-CreERT2 transgenic piglet (c), and brain organ (d), which were used to isolate neural stem cells (NSCs). (C) Representative brightfield images of primary pGFAP-CreERT2 NSCs. Scale, 50 μm. (D) Reverse transcriptase-PCR analysis was performed using genomic DNA isolated from the pGFAP-CreERT2 NSCs. (E) eGFP expression in pGFAP-CreERT2 NSCs was assessed by flow cytometry. Independent experiments were replicated at least three times

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