Table 4 Table showing semiquantitative scoring for histopathology examination
From: Comparison of hock- and footpad-injection as a prostate adenocarcinoma model in rats
Organ | Scoring | Description |
|---|---|---|
Liver | 0 | – |
1 | mild focal fatty infiltration around central vein(s) | |
2 | mild to moderate fatty infiltration around central vein(s) and in portal area, sinusoid dilatation | |
3 | severe fatty infiltration around central vein and in portal area, necrosis (hepatocellular dissociation) | |
Spleen | 0 | – |
1 | activated follicles, increased hemosiderosis | |
2 | increase of red and white pulp (follicles etc.), | |
3 | neutrophil granulocytes in red pulp, necrosis, fibrosis | |
Lymphnodes | 0 | – |
1 | secondary follicle activation, cortex vascular lesions (dilation of blood and/or lymphatic vessels) | |
2 | Localized: Secondary follicle activation, follicular depletion (atrophy), lymphatic sinus ectasia,medullar and cortex vascular lesions (dilation of blood and/or lymphatic vessels), macrophage hyperplasia (cortical-sinus) | |
3 | Whole organ (Cortex, Paracortex, Medulla): Secondary follicle activation, follicular depletion (atrophy), lymphatic sinus ectasia,medullar and cortex vascular lesions (dilation of blood and/or lymphatic vessels), macrophage hyperplasia (cortical and medullary sinus) | |
Kidneys | 0 | – |
1 | single protein liquid in proximal tubuli | |
2 | generalized protein liquid in proximal tubuli, interstitial nephritis, swollen mesangial cells | |
3 | necrosis, tubulonephrose, atrophic glomeruli, tubular cysts, papillar necrosis, fibrosis | |
Brain | 0 | – |
1 | severe hyperemia and congestion, oedema (widen “Virchof-Robin” space) | |
2 | additional gliosis, single neuron necrosis (shrunken hypereosinophilic neurons) | |
3 | additional severe gliosis/astrocytosis, neuronal necrosis, encephalitis, status spongiosus (severe oedema), lost of myelin sheath (demyelination) | |
Lung | 0 | – |
1 | severe hyperemia and congestion, alveolar oedema (liquid in alveoli with cellular reaction) | |
2 | additional some inflammatory cells in alveoli/interstiitum (macrophages, lymphocytes, neutrophils) | |
3 | additional multiple inflammatory cells in alveoli/interstiitum (macrophages, lymphocytes, neutrophils), proliferation of bronchial epithelium, bronchitis | |
Heart | 0 | – |
1 | severe hyperemia and congestion | |
2 | additional single perivascular inflammatory infiltrates and degeneration of myocytes | |
3 | additional myocard necrosis (focal and multifocal), deposits within vascular wall | |
Adrenal gland | 0 | – |
1 | severe hyperemia and congestion | |
2 | mild cortex/medulla activation (nodular hyperplasia), hypertrophy | |
3 | severe cortex/medulla activation (nodular hyperplasia), hypertrophy | |
Muscle | 0 | – |
1 | severe hyperemia and congestion, interstitial oedema, mild satelitosis | |
2 | additional swelling of the myocytes, degeneration (centraliation of myocytes nuclei), focla loss of striation, hypereosinophilic myocytes | |
3 | additional necrosis, inflammation, fatty infiltration | |
Tumor | 0 | – |
1 | tumor focus in primary organ with local invasion (infiltrative growth) | |
2 | tumor focus in primary organd with invasion and compression of adjacent tissue, mitosis per HPF40x (1–2), metastases in local lymph node (popliteal LNN) | |
3 | mitosis per HPF40x (3–7), vascular invasion (lymphatics, blood vessels), necrosis, cellular apoptosis, metastases lymph nodes (additional to popliteal Lnn) and other organs |