Table 1 Preliminary characterization results for the coagulase-negative Staphylococcus isolates recovered from bovine intramammary infections
Isolate reference (farm of origin) | Phenotyping assays | Multiplex PCR 1 | Species-level identification of CoNS isolates | Antimicrobial susceptibility testing | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Coagulase production | Pastorex StaphPlus test | 16S rRNA genus-specific target | S. aureus nuc gene | mecA gene | luk S/F gene | Bovine M-PCRa | MALDI-TOF MS (score)b | tuf gene sequencing | Penicillin (10 IU) | Ampicillin (10 μg) | Amoxicillin/Clavulanate (20 μg / 10 μg) | Cefoxitin (30 μg) | Cephalothin (30 μg) | Tetracycline (30 μg) | Trimethoprim/Sulfamethoxazole (1.25 μg / 23.75 μg) | Moxifloxacin (30 μg) | Clindamycin (2 μg) | Erythromycin (15 μg) | Gentamicin (10 μg) | Streptomycin (10 μg) | |
AN3 (commercial farm A) | (−) | (−) | (+) | (−) | (−) | (−) | (−) | No reliable identification | c | R | R | S | S | S | S | S | S | S | S | S | S |
AN19 (commercial farm A) | (−) | (−) | (+) | (−) | (−) | (−) | (−) | No reliable identification | c | S | S | S | S | S | S | S | S | S | S | S | S |
AN22 (commercial farm A) | (−) | (−) | (+) | (−) | (−) | (−) | (−) | No reliable identification | c | S | S | S | S | S | S | S | S | S | S | S | S |
AN25 (commercial farm A) | (−) | (−) | (+) | (−) | (−) | (−) | (−) | No reliable identification | c | R | R | S | S | S | S | S | S | S | S | S | S |
BN7 (commercial farm B) | (−) | (−) | (+) | (−) | (−) | (−) | (−) | S. lugdunensis (1.845 and 1.727) | c | R | R | S | S | S | S | S | S | S | S | S | S |
FN2 (commercial farm F) | (−) | (−) | (+) | (−) | (−) | (−) | (−) | No reliable identification | d | S | S | S | S | S | S | S | S | S | S | S | S |