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Table 1 Preliminary characterization results for the coagulase-negative Staphylococcus isolates recovered from bovine intramammary infections

From: Identification and characterization of Staphylococcus devriesei isolates from bovine intramammary infections in KwaZulu-Natal, South Africa

Isolate reference (farm of origin) Phenotyping assays Multiplex PCR 1 Species-level identification of CoNS isolates Antimicrobial susceptibility testing
Coagulase production Pastorex StaphPlus test 16S rRNA genus-specific target S. aureus nuc gene mecA gene luk S/F gene Bovine M-PCRa MALDI-TOF MS (score)b tuf gene sequencing Penicillin (10 IU) Ampicillin (10 μg) Amoxicillin/Clavulanate (20 μg / 10 μg) Cefoxitin (30 μg) Cephalothin (30 μg) Tetracycline (30 μg) Trimethoprim/Sulfamethoxazole (1.25 μg / 23.75 μg) Moxifloxacin (30 μg) Clindamycin (2 μg) Erythromycin (15 μg) Gentamicin (10 μg) Streptomycin (10 μg)
AN3 (commercial farm A) (−) (−) (+) (−) (−) (−) (−) No reliable identification c R R S S S S S S S S S S
AN19 (commercial farm A) (−) (−) (+) (−) (−) (−) (−) No reliable identification c S S S S S S S S S S S S
AN22 (commercial farm A) (−) (−) (+) (−) (−) (−) (−) No reliable identification c S S S S S S S S S S S S
AN25 (commercial farm A) (−) (−) (+) (−) (−) (−) (−) No reliable identification c R R S S S S S S S S S S
BN7 (commercial farm B) (−) (−) (+) (−) (−) (−) (−) S. lugdunensis (1.845 and 1.727) c R R S S S S S S S S S S
FN2 (commercial farm F) (−) (−) (+) (−) (−) (−) (−) No reliable identification d S S S S S S S S S S S S
  1. aThe bovine M-PCR assay comprised of primers specific for: S. chromogenes, S. epidermidis, S. haemolyticus, S. simulans, S. warneri and S. xylosus
  2. bMALDI-TOF MS interpretation: 2.00 to 2.299 = secure genus identification, probably species identification; 1.70 to 1.99 = probable genus identification; < 1.7 = not a reliable identification
  3. cAmplicon sequence showed 99–100% similarity with data in GenBank (EU571021)
  4. dAmplicon showed 97% similarity to S. haemolyticus; (+) = positive; (−) = negative; S = sensitive; R = resistant