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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Role of miR29c in goose fatty liver is mediated by its target genes that are involved in energy homeostasis and cell growth

Fig. 3

Prediction and validation of goose miR29c. a The number of goose miR29c target genes that were predicted by different online software programs. Col3a1, Sgk1, Insig1, DDX3X, PPM1D and TNFAIP3 were identified by multiple databases. b Expression of these genes in goose fatty liver vs. normal liver. Expression is presented as the fold change over the control, n = 5. c The sketch illustrates that the seed sequence of mature miR29c matches the 3’ UTR sequences of goose Col3a1, Sgk1 and Insig1. d Luciferase activity assay demonstrating that Col3a1, Sgk1 and Insig1 are the target genes of goose miR29c. Compared to negative control oligonucleotides (NC), miR29c mimics showed significantly reduced relative luciferase activity in CHO cells transfected with the PhRL-TK reporter vector and the pMIR-REPORT luciferase vector containing the 3’ UTR sequence of miR29c target genes, n = 4. All of the data are shown as the means ± SEM. *, ** denote p < 0.05, 0.01 vs. control, respectively

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