Fig. 9

Effect of TAZ or YAP knockdown plus and minus TGFβ on transwell migration of canine OSA cell lines. Representative images of migrated cells stained with 0.1% crystal violet taken with 10X objective for primary cell lines (a) and metastatic cell lines (b). Graphs depict the absorbance values of migrated of cells, as determined by extraction of crystal violet dye from transwells using 10% acetic acid and spectrophotometer reading at 590 nm. Readings were first blank-corrected to insert containing no cells. Error bars depict average ± SEM from at least three independent experiments plated in duplicate. Asterisks depict statistical differences as determined by a two-way ANOVA with post-hoc Tukey-Kramer, * indicates p < 0.05, ** indicates p < 0.010, *** indicates p < 0.0010. TAZ significantly reduced migration in the OVC-cOSA-31 and D17 cell lines, while YAP knockdown reduced migration in OVC-cOSA-75 and D17 cell lines