Fig. 7

Effect of TAZ or YAP knockdown plus and minus TGFβ on cell proliferation of canine OSA cell lines. Representative immunofluorescence images of the mitotic marker phosphor-histone H3 (pHH3) taken at 20X objective lens for primary tumour-derived cell lines (a), and metastatic cell lines (b). Graphs depict the relative percentage of pHH3 positivity cells, as determined by dividing the number of pHH3 cells by the number of cells in the field of view. Five images were taken per experimental group and averaged. Error bars depict average ± SEM from three independent experiments. Scale bar = 100 μm. Asterisks depict statistical differences as determined by a two-way ANOVA with post-hoc Tukey-Kramer, * indicates p < 0.05, ** indicates p < 0.010, *** indicates p < 0.0010