Fig. 5

All canine OSA cell lines express TGFβRI, TGFβRII, respond to TGFβ1 treatment and have basal levels of Hippo mediators TAZ and YAP. Cells were serum starved for 6 h and either stimulated with 5 ng/mL TGFβ1 (+) or not (−) for 24 h. Representative immunoblots and densitometry analysis showing the levels of TGFβRI and TGFβRII (a), pSmad3 and Smad 3 (b), pSmad2 and Smad2 (c) and TAZ and YAP (d), n = 3. Phospho-protein levels were normalized to native protein levels, and all native protein levels were normalized to β actin (loading control) and compared to the control (−) to determine relative differences. Independent t-test was used to determine significant differences between control and treated groups, * indicates p < 0.05, ** indicates p < 0.010, error bars depict average ± SEM