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Table 2 Comparative performance of serotype-specific RT-LFD-RPA and rRT-PCR assays for detection of suspected clinical specimens and serotyping of FMDV

From: Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1

 

LFD-RPA

 

Real-time

(qPCR)

 

Samples name

A

Asia 1

O

FMDV positive

FMDV Negative

A

Asia 1

O

FMDV positive

FMDV Negative

vesicular material

7

3

15

25

5

7

3

15

25

5

nasal swab

6

2

5

13

7

6

2

5

13

7

saliva

5

3

6

14

15

5

3

6

14

15

oesophageal pharyngeal fluid

6

4

4

14

3

6

4

4

14

3

blood

4

2

3

9

7

4

2

3

9

7

aerosol

4

3

3

10

4

3

3

2

8

6

Total

32

17

36

85

41

31

17

35

83

43