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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Development and evaluation of serotype-specific recombinase polymerase amplification combined with lateral flow dipstick assays for the diagnosis of foot-and-mouth disease virus serotype A, O and Asia1

Fig. 1

Screening of the primers/probes for the FMDV serotype-specific RPA-LFD assay. a Agarose gel electrophoresis and LFD detection of RT-RPA products amplified with different primer and probe sets of FMDV serotype A. Lane M was DNA Marker DL1000. A1 to A9 were different primer and probe sets. A6: the optimal primer and probe set, and the estimated size of the RPA amplified fragment were 346 bp and 334 bp. A10: negative control, (DNase-free water). A11: positive control (supplied by Twist Amp nfo kit). b Agarose gel electrophoresis and LFD detection of RT-RPA products amplified with different primers/probe sets of FMDV serotype Asia 1. As2 to As10 were different primer and probe sets. As9: the optimal primer/probe set, and the estimated size of the RPA amplified fragment were 286 bp and 182 bp. As1: negative control (DNase-free water). As10: positive control (supplied by Twist Amp nfo kit). c Agarose gel electrophoresis and LFD detection of RT-RPA products amplified with different primers/probe sets of FMDV serotype O. O1 to O9 were different primer and probe sets. O6: the optimal primer/probe set, and the estimated size of the RPA amplified fragment were 231 bp and 190 bp. O10: negative control (DNase-free water). O11: positive control (supplied by Twist Amp nfo kit)

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BMC Veterinary Research

ISSN: 1746-6148

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