Changes in ruminal and reticular pH and bacterial communities in Holstein cattle fed a high-grain diet

BMC Veterinary Research

Table 2 Relative abundance (% of total sequences) of the major bacterial phyla and genera

Items	HAY Period		CON Period		SEM	P value
Items	Rumen (n = 4)	Reticulum (n = 4)	Rumen (n = 4)	Reticulum (n = 4)	SEM	Period (P)	Location (L)	P x L
Phylum
Firmicutes	37.80	39.18	62.85	60.79	5.54	0.002	0.962	0.706
Bacteroidetes	30.26	31.11	10.84	14.19	5.89	0.014	0.762	0.822
Actinobacteria	3.36	1.70	4.72	3.46	1.16	0.114	0.401	0.814
Genus
Prevotella	27.20	27.15	5.29	8.56	4.84	0.006	0.766	0.746
Ruminococcus	3.56	4.09	19.96	14.97	3.28	0.019	0.626	0.541
Clostridium	3.76	3.59	13.37	14.22	2.16	0.006	0.910	0.839
Butyrivibrio	3.05	3.28	11.95	12.13	3.19	0.117	0.931	0.977
Eubacterium	3.24	3.75	1.41	1.98	0.34	0.025	0.027	0.912
Oscillibacter	3.84	3.40	0.34	0.49	0.46	0.001	0.825	0.608
unclassified Clostridiales	2.85	2.71	0.81	0.97	0.94	0.258	0.426	0.692
Barnesiella	0.88	1.22	3.25	3.97	1.11	0.077	0.743	0.879
Acetivibrio	1.89	1.66	1.11	1.12	0.87	0.716	0.459	0.996
unclassified Lachnospiraceae	1.95	2.01	0.28	0.30	0.59	0.173	0.593	0.431
Succiniclasticum	1.70	1.31	0.65	0.90	0.26	0.037	0.800	0.280
Olsenella	0.67^a	0.63^b	2.31^ac	1.57^bc	0.39	0.012	0.144	0.024
unclassified Clostridiaceae	0.95	1.09	0.96	1.53	0.31	0.800	0.265	0.552
Bacteroides	0.91	1.12	1.36	0.57	0.49	0.519	0.985	0.782

^{a-a, b-b, c-c} means within a row, same superscripts differ significantly (P < 0.05)

ISSN: 1746-6148