Table 1 Primer list for RT-qPCR
From: Notch2 signal is required for the maintenance of canine hemangiosarcoma cancer stem cell-like cells
Primer | Sequence | Gene ID and references | Efficiency (%) | |
|---|---|---|---|---|
Forward primer | Reverse primer | |||
ERG | CAAACATGACCACGAACGAG | AGGCCGTATTCTTTCACTGC | ENSCAFG 00000009912 | 98.4 |
PROCR | GCAGGAACACAATGCTTCAA | AAGATGCCTACAGCCACACC | ENSCAFG 00000007945 | 95.5 |
SOX18 | TGAACGCCTTCATGGTGTG | GGCGTCAGCTCCTTCCAC | ENSCAFG 00000029278 | 94.5 |
FLI1 | TACTGAACAAAGGCCCCAAC | ACTGTCCGAGAGAAGCTCCA | ENSCAFG 00000032412 | 96.9 |
NOTCH1 | TACCGGCCAGAACTGTGAGGAGAA | GGAGGGCAGCGGCAGTTGTAAGTA | ENSCAFG 00000019633 | 93.6 |
NOTCH2 | TCGGGATAGCTATGAGCCCT | GGCATGTTGCTTTCCCCAAC | ENSCAFG 00000010476 | 93.6 |
NOTCH3 | ACAACTGCCAGTGTCCTCCT | GTCCAGCCATTGACACACAC | ENSCAFG 00000016107 | 93.9 |
NOTCH4 | AAGCCCTGTCCACACAATTC | CTGGCATAGGGAAGAAGCTG | ENSCAFG 00000000791 | 95.4 |
HEY1 | GCGCGGATGAGAATGGAAAC | GTCGGCGCTTCTCAATGATG | ENSCAFG 00000008391 | 95.3 |
HEY2 | CGGCGAGATCGGATAAATAA | CGCGTCGAAGTAGCCTTTAC | XM_541232.5 ENSCAFG 00000032212 | 96.4 |
HES1 | CATCCAAGCCTATCATGGAGA | GTTCCGGAGGTGCTTCACT | Dailey DD et al. | 95.5 |
HES6 | CAGGCCAAGCTGGAGAAC | GCATGCACTGGATGTAGCC | ENSCAFG 00000012428 | 96.5 |
NRARP | TGAAGCTGCTGGTCAAGTTC | CTTGGCCTTGGTGATGAGAT | ENSCAFG 00000019445 | 94.5 |
FCER2 | GAGGAGGTGGAGAAGCTGTG | CCTCGCCGAAGTAGTAGCAC | ENSCAFG 00000030055 | 97.5 |
GAPDH | ATTCCACGGCACAGTCAAG | TACTCAGCACCAGCATCACC | ENSCAFG 00000015077 | 99.5 |
ACTB | CCAGCAAGGATGAAGATCAAG | TCTGCTGGAAGGTGGACAG | ENSCAFG 00000016020 Peters IR. et al. | 98.8 |
HMBS | TCACCATCGGAGCCATCT | GTTCCCACCACGCTCTTCT | ENSCAFG 00000012342 Peters IR. et al | 95.8 |
RPL13A | GCCGGAAGGTTGTAGTCGT | GGAGGAAGGCCAGGTAATTC | ENSCAFG 00000029892 Peters IR. et al | 98.2 |
RPL32 | TGGTTACAGGAGCAACAAGAAA | GCACATCAGCAGCACTTCA | ENSCAFG 00000004871 Peters IR. et al | 95.9 |
HPRT1 | CACTGGGAAAACAATGCAGA | ACAAAGTCAGGTTTATAGCCAACA | ENSCAFG 00000018870 Peters IR. et al | 95.7 |
B2M | ACGGAAAGGAGATGAAAGCA | CCTGCTCATTGGGAGTGAA | ENSCAFG 00000013633 Peters IR. et al | 98.2 |
YWHAZ | CGAAGTTGCTGCTGGTGA | TTGCATTTCCTTTTTGCTGA | ENSCAFG 00000000580 Peters IR. et al | 93.4 |
TBP | ATAAGAGAGCCCCGAACCAC | TTCACATCACAGCTCCCCAC | ENSCAFG 00000004119 Peters IR. et al | 97.3 |