Molecular characterization of three novel perforins in common carp (Cyprinus carpio L.) and their expression patterns during larvae ontogeny and in response to immune challenges

Table 1 Intron-exon junctions and flanking sequences of the CcPRF1, CcPRF2 and CcPRF3

Exon no.	Position in DNA	Exon size (bp)	Splice donor	Splice acceptor	Intron size (bp)
CcPRF1–1	1–52	52	gtaagtgaaa	atctcagcag	443
2	496–1033	538	gtaagactta	ctgtcttcag	89
3	1123–1261	139	gtaagccaaa	aatctgttag	83
4	1345–1562	218	gtttgtgctt	tttctacaag	530
5	2093–3003	911
CcPRF2–1	1–27	27	gtaaatatca	tctctttcag	1127
2	1155–1670	516	gtaagttctg	ctttcatcag	2475
3	4146–4416	271	gtgagtgggc	gggcgtgaag	391
4	4808–5406	599	gtaagtggtt	cattttacag	97
5	5504–6046	543
CcPRF3–1	1–69	69	gtaagaaact	ttcactacag	603
2	673–838	166	gttcttatgt	gagcgaaaag	85
3	924–1275	352	gttatttttc	ttctttatag	119
4	1395–2761	1367

ISSN: 1746-6148