BMC Veterinary Research

Table 2 Comparison of FMDV LFS RT-RPA with real-time RT-PCR assays performed on RNA extracts from the virus strains, clinical samples, spiked serum samples and samples from healthy controls

From: Visual and equipment-free reverse transcription recombinase polymerase amplification method for rapid detection of foot-and-mouth disease virus

Sample type	Sample name	LFS RT-RPA	real-time RT-PCR(Ct)
Virus strains	FMDV type O	+	18.95
	FMDV type A	+	20.63
	FMDV type Asia1	+	20.37
Clinical	4	+	23.45
samples	28	+	32.58
	124	+	26.78
	125	+	30.42
	126	+	30.24
	131	+	24.26
	133	+	30.76
	140	+	33.75
	208	+	25.11
	209	-	35.85
	213	-	36.96
	217	+	32.10
Spiked	Swine serum 1	+	23.47
serum samples	(1:1)
	Swine serum 2	+	25.75
	(1:10)
	Swine serum 3	+	26.41
	(1:20)
	Swine serum 4	+	28.45
	(1:40)
	Swine serum 5	+	33.24
	(1:80)
	Swine serum 6	+	34.45
	(1:100)
	Swine serum 7	-	37.15
	(1:200)
	Swine serum 8	-	>40.00
	(1:400)
	Bovine serum 1	+	23.08
	(1:1)
	Bovine serum 2	+	25.51
	(1:10)
	Bovine serum 3	+	26.64
	(1:20)
	Bovine serum 4	+	29.56
	(1:40)
	Bovine serum 5	+	34.40
	(1:80)
	Bovine serum 6	+	35.14
	(1:100)
	Bovine serum 7	-	37.64
	(1:200)
	Bovine serum 8	-	>40.00
	(1:400)
Control samples	Swine serum	-	>40.00
	(9-20)
	Bovine serum	-	>40.00
	(9-20)

+ : positive; - : negative

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ISSN: 1746-6148

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