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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Visual and equipment-free reverse transcription recombinase polymerase amplification method for rapid detection of foot-and-mouth disease virus

Fig. 1

Diagram of LF probe and post-RPA detection with lateral flow strip. LF probe is typically 46–52 nucleotides long, at least 30 of which are placed 5′ to the THF site, and at least a further 15 nucleotides are located 3′ to the site. Detection of amplicons is accomplished by capture of tags with anti-FAM antibodies and biotin-ligand molecules generating a visual colored line on LFS

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