Sodium butyrate improves antioxidant stability in sub-acute ruminal acidosis in dairy goats

BMC Veterinary Research

Table 2 Primer sequences used for real-time PCR amplification of selected genes in the livers of lactating goats

Gene^a	Primers 5′ to 3’^b	Size(bp)	Accession ^c
SOD1	ACACAAGGCTGTACCAGTGC TTCACATTGCCCAGGTCTCC	104	NM_001285550.1
SOD2	TCAATAAGGAGCAGGGACGC AGCAGGGGGATAAGACCTGT	85	XM_005684984.1
SOD3	ATCGACCCGAACGGTAACGC AGGACATAGAAGGGGTCTGCG	199	XM_005681678.1
CAT	ACAATGTCACTCAGGTGCGG TCTCACACAGGCGTTTCCTC	70	XM_005690077.1
GPx1	GTAGCATCGCTCTGAGGCAC TTGGCATTTTCCTGATGCCC	131	XM_005695962.1
GPx3	TTCGGTCTGGTCATTCTGGG CGAACATACTTGAGGGTGGC	95	XM_005683183.1
HMOX2	AGCTAGACAAAGGTGCCCTG CAGGATGCTGACAGGCAAAG	113	XM_005697930.1
MGST3	AGCTCACCAGAACACGTTGG GCCCAAACCAGAAGCTATGC	94	NM_001285615.1
NQO1	CATGGCTGTCAGAAAAGCACTG GACACAGTGACCTCCCATCC	120	XM_005692193.1
UGT1A1	TCCGGAGCAGAAAGCTATGG CCGAGTCTTTGGGTGACCAAG	160	XM_005678746.1
TXNRD1	TTTCGCTCAGTTTGCTCCAG TCCTGAGAAGCCTTCAGGGTC	200	XM_005680564.1
NF-kB	AGGTGGCGATCGTTGTTCTA TTGCCTTTGTTCTTCCTGCC	226	XM_00569893.1
NrF2	AGCCAGGTGAGATGGAACTG CCAGACTCCCTGTTTCGCTG	120	XM_005679848.2
MT2A	TCCTGCAAGAAGAGCTGCTG AACTGCACTTGTCCGAGGC	94	XM_005691999.1
SRXN1	GACACGATCCGGGAGAATCC GGTCTGAGAGGGTGGATTGG	175	XM_013968785.1
MT1A	ATAATAGCGCTCGGCTCCTG TTGGAGGAAAAGCGAGGTCC	98	XM_005692001.2
GAPDH	CCTGCCCGTTCGACAGATAG CCGTTCTCTGCCTTGACTGT	249	XM_005680968.2

^aSOD1 superoxide dismutase 1, SOD2 superoxide dismutase 2, SOD3 superoxide dismutase3, GPX1 glutathione peroxidase1, GPX3 glutathione peroxidase 3, CAT catalase, HMOX2 haem oxygenase 2, MGST3 microsomal glutathione S-transferase 3, MT1A metallothionein 1A, NQO1 NAD(P)H, quinone 1, UGT1A1 UDP glucuronosyltransferase 1 family, polypeptide A1, MT2A metallothionein 2A, TXNRD1 thioredoxin reductase 1, SRXN1 sulfiredoxin 1, GAPDH glyceraldehyde 3-phosphate dehydrogenase
^bThe first primer listed for each gene is the forward primer, and the second primer is the reverse primer
^cThe reference sequence numbers are given for primers whose source is the National Center for Biotechnology Information (NCBI) GenBank database (http://www.ncbi.nlm.nih.gov/genbank/)

ISSN: 1746-6148