Table 2 The primer sequences of caecal targeting total bacteria, Lactobacillus, Bifidobacteria, Enterococcus, Enterobacteriaceae, E.coli, and Salmonella
| Microorganism | Primer | Size of amplified fragments (bp) | References |
|---|---|---|---|
| Lactobacillus |
F-5′-CATCCAGTGCAAACCTAAGAG-3′ R-5′-GATCCGCTTGCCTTCGCA-3′ | 341 | [42] |
| Enterococcus genus |
F-5′-CCCTTATTGTTAGTTGCCATCATT-3′ R-5′-ACTCGTTGTACTTCCCATTGT-3′ | 144 | [9] |
| Bifidobacterium |
F-5′- GGGTGGTAATGCCGGATG-3′ R-5′- TAAGCCATGGACTTTCACACC-3′ | 440 | [43] |
| Escherichia coli |
F-5′-GTGTGATATCTACCCGCTTCGC-3′ R-5′-AGAACGCTTTGTGGTTAATCAGGA-3′ | 82 | [42] |
| Enterobacteriace |
F- 5′-CAT TGACGTTACCCGCAGAAGAAGC-3′ R-5′-CTCTACGAGACTCAAGCTTGC-3′ | 195 | [42] |
| Total Salmonella |
F-5’-TCGTCATTCCATTACCTACC-3′ R-5’-AAACGTTGAAAAACTGAGGA-3′ | 119 | [44] |