Table 2 The primer sequences of caecal targeting total bacteria, Lactobacillus, Bifidobacteria, Enterococcus, Enterobacteriaceae, E.coli, and Salmonella
Microorganism | Primer | Size of amplified fragments (bp) | References |
|---|---|---|---|
Lactobacillus | F-5′-CATCCAGTGCAAACCTAAGAG-3′ R-5′-GATCCGCTTGCCTTCGCA-3′ | 341 | [42] |
Enterococcus genus | F-5′-CCCTTATTGTTAGTTGCCATCATT-3′ R-5′-ACTCGTTGTACTTCCCATTGT-3′ | 144 | [9] |
Bifidobacterium | F-5′- GGGTGGTAATGCCGGATG-3′ R-5′- TAAGCCATGGACTTTCACACC-3′ | 440 | [43] |
Escherichia coli | F-5′-GTGTGATATCTACCCGCTTCGC-3′ R-5′-AGAACGCTTTGTGGTTAATCAGGA-3′ | 82 | [42] |
Enterobacteriace | F- 5′-CAT TGACGTTACCCGCAGAAGAAGC-3′ R-5′-CTCTACGAGACTCAAGCTTGC-3′ | 195 | [42] |
Total Salmonella | F-5’-TCGTCATTCCATTACCTACC-3′ R-5’-AAACGTTGAAAAACTGAGGA-3′ | 119 | [44] |