Fig. 1

Preparation and infiltration of Plasma Rich in Growth Factors (PRGF) together with Adipose-Deived Mesenchymal Stem Cells (ASCs) isolation, culture and differentiation. a Blood extraction from auricular artery of each rabbit (5 ml). b Blood collection in vacutainer sodium citrate 3,8% tubes (BTI Biotechnology institute, Álava, Spain). c Perilesional PRGF infiltration into corresponding cutaneous wounds. d Harvested adipose tissue from donor rabbit. e, f Morphology of ASCs cultured in vitro, migration and wound closure capacity in vitro. g, h, i Multipotent ability of ASCs by induced differentiation of adipocytes (G) is shown by Oil Red staining, scale bar: 15 μm osteoblasts (H) showing calcium accumulation by Alizarin Red staining, scale bar: 100 μm; and chondrocytes (I) showing high content of condroitinsulfate enriched matrix stained by Alcian Blue, scale bar: 100 μm