Fig. 3

Western blotting analysis of VEGF, vWF, bcl-2 and Ki67 protein expression in equine sarcoids (S) and normal skin samples (N). a VEGF was expressed in all the analysed samples, with higher protein levels in S29 and S30. Hela whole cell lysate was run along with equine samples as positive control. The membrane was re-probed for β-actin to allow normalization. b Densitometric values were measured and expressed as VEGF/actin ratio. c vWF was detectable in sarcoids but not in normal skin samples. Saos-2 whole cell lysate was run along with equine samples as positive control. The Saos-2 box is cut from the same membrane at a different exposure time and properly aligned according to the molecular standard loaded onto the gel. The membrane was re-probed for β-actin to allow normalization. d Densitometric values were measured and expressed as vWF/actin ratio. e bcl-2 was expressed at variable levels in the analysed samples. Hela whole cell lysate was run along with equine samples as positive control. The membrane was re-probed for β-actin to allow normalization. f Densitometric values were measured and expressed as bcl-2/actin ratio. g Variable expression of Ki67 protein in the analysed samples. Hela whole cell lysate was run along with equine samples as positive control. The Hela box is cut from the same membrane at a different exposure time and properly aligned according to the molecular standard loaded onto the gel. The membrane was re-probed for β-actin to allow normalization. h Densitometric values were measured and expressed as Ki67/actin ratio