|
Flocks
|
Sheep clinical status (foot lesions)
|
No. sheep tested
|
aprV2 PCR Run/Cut-off
|
Culture Gelatinase Thermostable (Virulent) vs aprV2 PCR
|
Percentage positive results
|
|---|
|
Concordant results
|
Culture +ve
|
PCR + ve
|
p value
|
Kappa
|
Culture
|
aprV2 PCR
|
|---|
|
+ve
|
-ve
|
PCR -ve
|
Culture -ve
|
|---|
|
12 to 24
|
+ve
|
135
|
Run 1 / 40
|
31
|
17
|
0
|
87
|
< 0.0001
|
0.082
|
23.0%
|
87.4%
|
|
12 to 24
|
+ve
|
135
|
Run 1 / 35
|
31
|
20
|
0
|
84
|
< 0.0001
|
0.1
|
23.0%
|
85.2%
|
|
12 to 14
|
-ve
|
35
|
Run 1 / 40
|
3
|
16
|
0
|
16
|
0.0002
|
0.146
|
8.6%
|
54.3%
|
|
12 to 14
|
-ve
|
35
|
Run 2 / 40
|
3
|
12
|
0
|
20
|
< 0.0001
|
0.093
|
8.6%
|
65.7%
|
|
12 to 14
|
-ve
|
35
|
Run 1 / 35
|
3
|
20
|
0
|
12
|
0.0015
|
0.222
|
8.6%
|
42.9%
|
|
12 to 14
|
-ve
|
35
|
Run 2 / 35
|
3
|
19
|
0
|
13
|
0.0009
|
0.2
|
8.6%
|
45.7%
|
|
12 to 14
|
-ve & + ve
|
72
|
Run 1 / 40
|
9
|
20
|
0
|
43
|
< 0.0001
|
0.104
|
12.50%
|
72.20%
|
|
12 to 14
|
-ve & + ve
|
72
|
Run 2 / 40
|
9
|
16
|
0
|
47
|
< 0.0001
|
0.078
|
12.50%
|
77.80%
|
|
12 to 14
|
-ve & + ve
|
72
|
Run 1 / 35
|
9
|
27
|
0
|
36
|
< 0.0001
|
0.158
|
12.50%
|
62.50%
|
|
12 to 14
|
-ve & + ve
|
72
|
Run 2 / 35
|
9
|
27
|
0
|
36
|
< 0.0001
|
0.158
|
12.50%
|
62.50%
|
|
12 to 24
|
-ve & + ve
|
170
|
Run 1 / 40
|
34
|
33
|
0
|
103
|
< 0.0001
|
0.114
|
20.0%
|
80.6%
|
|
12 to 24
|
-ve & + ve
|
170
|
Run 1 / 35
|
34
|
40
|
0
|
96
|
< 0.0001
|
0.143
|
20.0%
|
76.5%
|
- The p-value for McNemar’s test for independence between culture gelatinase thermostable (Virulent) and aprV2 PCR result is shown. Detection rates of both culture and rtPCR are presented as percentage positive results
