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Table 5 Results from two cut offs of Ct 40 and 35 from the aprB2 rtPCR and aprV2 rtPCR obtained from two runs, in 72 sheep randomly sampled from 3 flocks considered having virulent footrot

From: Assessment of a rtPCR for the detection of virulent and benign Dichelobacter nodosus, the causative agent of ovine footrot, in Australia

Foot lesion

Cut off 40

Cut off 35

Run 1 + ve

Run 1 -ve

Run 2 + ve

Run 2 -ve

Run 1 + ve

Run 1 -ve

Run 2 + ve

Run 2 -ve

aprB2

 Positive

9

28

8

29

7

30

7

30

 Negative

2

33

1

34

1

34

1

34

 Overall % positive

 

15.30%

 

12.50%

 

11.10%

 

11.10%

 McNemars two tailed p value

 

< 0.0001

 

< 0.0001

 

< 0.0001

 

< 0.0001

aprB2 cut off agreement

 Run 2 + ve

8

1

  

8

0

  

 Run 2 -ve

3

60

  

0

64

  

 Kappa

0.768

“good”

  

1

“perfect”

  

aprV2

 Positive

33

4

33

4

30

7

29

8

 Negative

19

16

23

12

15

20

16

19

 Overall % positive

 

72.20%

 

77.80%

 

62.50%

 

62.50%

 McNemars two tailed p value

 

0.0035

 

0.0005

 

0.1356

 

0.153

aprV2 cut off agreement

 Run 2 + ve

51

5

  

44

1

  

 Run 2 -ve

1

15

  

1

26

  

 Kappa

0.779

“good”

  

0.941

“very good”

  
  1. The p-value for McNemar’s test for independence between lesion score and rtPCR result is shown along with kappa statistic for agreement between rtPCR runs
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BMC Veterinary Research

ISSN: 1746-6148

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