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Table 1 Descriptive characteristics of 11 Australian sheep flocks considered free of virulent footrot were sampled between June ‘15 and August ‘15 for evaluation of the specificity of an rtPCR for detection of virulent (aprV2) and benign (aprB2) protease genes of D. nodosus

From: Assessment of a rtPCR for the detection of virulent and benign Dichelobacter nodosus, the causative agent of ovine footrot, in Australia

Flock ID

No. animals sampled

Sampling date

Flock origin (shire or city)

Breed

Age

Sex

Comments/Flock history

1

18

02.06.2015

City of Broken Hill, NSW

Merino

Lambs

Mixed

Abbatoir line.

2

18

02.06.2015

Blayney Shire, NSW

Mixed

Mixed

Mixed

Abbatoir line.

3

19

02.06.2015

Shire ofArarat, Vic

Mixed

Rams

Male

Abbatoir line.

4

18

02.06.2015

Shire of Ararat, Vic

Merino

Mixed

Female

Abbatoir line.

5

19

02.06.2015

Shire of Ararat, Vic

Merino

Lambs

Mixed

Abbatoir line.

6

18

02.06.2015

City of Wagga Wagga, NSW

Merino

Ewes

Female

Abbatoir line.

7

18

02.06.2015

Southern Grampians Shire, Vic

Crossbreed

Ewes

Female

Abbatoir line.

8

55

16.06.2015

Yarra Ranges Shire, Vic

Coopworth crosses

Ewes

Female

Farm has had previous intermittent lameness, footrot has not been confirmed as the cause.

9

81

01.07.2015

Strathbogie Shire, Vic

Merino

Wethers

Male

Farm has no history of footrot, but lameness occasionally observed. Sheep footbathed late 2014.

10

54

17.07.2015

Wellington Shire, Vic

Merino

Wethers

Male

Well managed merino stud, no history of footrot. Wether had strayed into adjoining properties and had been cought and shorn 2 days prior to sampling.

11a

27

05.08.2015

East Gippsland Shire, Vic

Merino

Ewes

Female

Virulent footrot first introduced in the 80’s, treated by footbathing. Second footrot introduced in 1995; eradicated by footbathing, antibiotic regime and culling. A closed flock currently. No footbathing for ≥ 10 years.

  1. a Flock 11 has been excluded from specificity and sensitivity calculations because of its history