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Table 1 Descriptive characteristics of 11 Australian sheep flocks considered free of virulent footrot were sampled between June ‘15 and August ‘15 for evaluation of the specificity of an rtPCR for detection of virulent (aprV2) and benign (aprB2) protease genes of D. nodosus

From: Assessment of a rtPCR for the detection of virulent and benign Dichelobacter nodosus, the causative agent of ovine footrot, in Australia

Flock ID No. animals sampled Sampling date Flock origin (shire or city) Breed Age Sex Comments/Flock history
1 18 02.06.2015 City of Broken Hill, NSW Merino Lambs Mixed Abbatoir line.
2 18 02.06.2015 Blayney Shire, NSW Mixed Mixed Mixed Abbatoir line.
3 19 02.06.2015 Shire ofArarat, Vic Mixed Rams Male Abbatoir line.
4 18 02.06.2015 Shire of Ararat, Vic Merino Mixed Female Abbatoir line.
5 19 02.06.2015 Shire of Ararat, Vic Merino Lambs Mixed Abbatoir line.
6 18 02.06.2015 City of Wagga Wagga, NSW Merino Ewes Female Abbatoir line.
7 18 02.06.2015 Southern Grampians Shire, Vic Crossbreed Ewes Female Abbatoir line.
8 55 16.06.2015 Yarra Ranges Shire, Vic Coopworth crosses Ewes Female Farm has had previous intermittent lameness, footrot has not been confirmed as the cause.
9 81 01.07.2015 Strathbogie Shire, Vic Merino Wethers Male Farm has no history of footrot, but lameness occasionally observed. Sheep footbathed late 2014.
10 54 17.07.2015 Wellington Shire, Vic Merino Wethers Male Well managed merino stud, no history of footrot. Wether had strayed into adjoining properties and had been cought and shorn 2 days prior to sampling.
11a 27 05.08.2015 East Gippsland Shire, Vic Merino Ewes Female Virulent footrot first introduced in the 80’s, treated by footbathing. Second footrot introduced in 1995; eradicated by footbathing, antibiotic regime and culling. A closed flock currently. No footbathing for ≥ 10 years.
  1. a Flock 11 has been excluded from specificity and sensitivity calculations because of its history