Table 1 Checkerboard titration of the recombinant protein S1
OD450 ratio (P/N) | Dilution of serum sample | |||||
|---|---|---|---|---|---|---|
P/N | 1:20 | 1:40 | 1:80 | 1:160 | 1:320 | |
Concentrations of antigen (μg/mL) | 10 | 1.233/0.185 | 0.980/0.100 | 0.730/0.082 | 0.551/0.052 | 0.401/0.041 |
6.665 | 9.80 | 8.902 | 10.596 | 9.780 | ||
7.5 | 1.118/0.150 | 0.942/0.092 | 0.631/0.075 | 0.459/0.047 | 0.309/0.029 | |
7.453 | 10.239 | 8.413 | 9.766 | 10.655 | ||
5.0 | 1.102/0.141 | 0.854/0.077 | 0.591/0.067 | 0.388/0.042 | 0.228/0.022 | |
7.816 | 11.091 | 8.821 | 9.238 | 10.364 | ||
2.5 | 1.032/0.120 | 0.832/0.073 | 0.547/0.063 | 0.335/0.040 | 0.195/0.019 | |
8.600 | 11.397 | 8.683 | 8.375 | 10.263 | ||
1.0 | 0.927/0.112 | 0.753/0.071 | 0.473/0.057 | 0.316/0.037 | 0.183/0.017 | |
8.277 | 10.606 | 8.298 | 8.541 | 10.765 | ||
0.5 | 0.818/0.103 | 0.714/0.068 | 0.447/0.055 | 0.292/0.033 | 0.171/0.016 | |
7.942 | 10.500 | 8.127 | 8.848 | 10.688 | ||