Skip to main content

Table 1 Checkerboard titration of the recombinant protein S1

From: Development and application of an indirect ELISA for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike protein

OD450 ratio (P/N) Dilution of serum sample
P/N 1:20 1:40 1:80 1:160 1:320
Concentrations of antigen (μg/mL) 10 1.233/0.185 0.980/0.100 0.730/0.082 0.551/0.052 0.401/0.041
6.665 9.80 8.902 10.596 9.780
7.5 1.118/0.150 0.942/0.092 0.631/0.075 0.459/0.047 0.309/0.029
7.453 10.239 8.413 9.766 10.655
5.0 1.102/0.141 0.854/0.077 0.591/0.067 0.388/0.042 0.228/0.022
7.816 11.091 8.821 9.238 10.364
2.5 1.032/0.120 0.832/0.073 0.547/0.063 0.335/0.040 0.195/0.019
8.600 11.397 8.683 8.375 10.263
1.0 0.927/0.112 0.753/0.071 0.473/0.057 0.316/0.037 0.183/0.017
8.277 10.606 8.298 8.541 10.765
0.5 0.818/0.103 0.714/0.068 0.447/0.055 0.292/0.033 0.171/0.016
7.942 10.500 8.127 8.848 10.688