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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Development and application of an indirect ELISA for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike protein

Fig. 1

Amplification, SDS-PAGE and western blotting analysis of the recombinant protein S1. a RT-PCR amplification of the truncated S1 gene fragment. Lane M, DL2000 DNA marker. Lane 1 and 2, the truncated S1 gene fragment. b Identifiction of the recombinant expression plasmid 28a-S1 by double enzyme digestion. Lane M, DL5000 DNA marker. Lane 1, the recombinant expression plasmid 28a-S1 digested by BamH I/Sal I. c SDS-PAGE analysis of S1 protein. Lane M, prestained protein molecular weight standard. Lane 1, transformed cells of BL21/pET-28a(+) after IPTG induction for 6 h. Lane 2, transformed cells of BL21/28a-S1 after IPTG induction for 6 h. Lane 3, purified recombinant protein S1 by affinity chromatography of Ni-NTA spin column. d Western blotting analysis of S1 protein. Lane M, prestained protein molecular weight standard. Lane 1, E. coli BL21 with empty vector pET-28a(+) reacted with polyclonal mouse anti-PEDV antibody. Lane 2, purified S1 protein reacted with polyclonal mouse anti-PEDV antibody. A prominent band with the expected size 42 kDa appeared after incubation

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